9LX2

anti-CRISPR-associated protein, Aca11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	125
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 1
Unit cell lengths	24.060, 52.600, 58.290
Unit cell angles	105.55, 96.90, 100.56

Refinement procedure

Resolution	27.630 - 1.940
R-factor	0.2006
Rwork	0.199
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	3.169
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.630	2.009
High resolution limit [Å]	1.940	1.940
Number of reflections	17422	1833
<I/σ(I)>	12.48
Completeness [%]	88.3
Redundancy	3.6
CC(1/2)	0.997	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20% (w/v) PEG 3000, 0.1M Sodium citrate pH 5.2