9LX1

anti-CRISPR-associated protein, Aca7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2024-03-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	39.460, 39.460, 90.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.320 - 1.250
Rwork	0.188
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.934
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.320	1.295
High resolution limit [Å]	1.250	1.250
Number of reflections	22730	2169
<I/σ(I)>	52.51
Completeness [%]	96.9
Redundancy	18.8
CC(1/2)	1.000	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	32% (w/v) PEG 4000, 0.1M Tris pH 8.5, 0.2M Lithium sulfate