9LWX

Crystal structure of the Filamin A repeat 21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-02-04
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	37.694, 47.912, 50.360
Unit cell angles	78.42, 83.69, 90.10

Refinement procedure

Resolution	28.270 - 2.290
R-factor	0.2224
Rwork	0.220
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.820
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.270	2.370
High resolution limit [Å]	2.290	2.290
Number of reflections	13931	883
<I/σ(I)>	8
Completeness [%]	90.1
Redundancy	13
CC(1/2)	0.930	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Sodium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate, pH 6.5, 30% w/ Polyethylene glycol (PEG) 8,000