9LWV
Arginine bis-prenyltransferase DciF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.977, 94.242, 92.645 |
| Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
| Resolution | 47.370 - 2.600 |
| R-factor | 0.2561 |
| Rwork | 0.254 |
| R-free | 0.28570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.449 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.124 | 0.807 |
| Number of reflections | 37042 | 4493 |
| <I/σ(I)> | 7.9 | 1.4 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.7 | 4.4 |
| CC(1/2) | 0.995 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Tris-HCl (pH 8.5), 30% (w/v) PEG 4000 |
