9LVP
Crystal structure of estrogen related receptor-alpha DNA binding domain complexed with MPC1 promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.021, 66.021, 365.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.460 - 2.790 |
| R-factor | 0.22156 |
| Rwork | 0.219 |
| R-free | 0.27962 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.860 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.460 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.148 | 2.279 |
| Rmeas | 0.152 | 2.329 |
| Rpim | 0.032 | 0.477 |
| Total number of observations | 466397 | 71040 |
| Number of reflections | 21207 | 3039 |
| <I/σ(I)> | 16.4 | 2.1 |
| Completeness [%] | 98.8 | |
| Redundancy | 22 | 23.4 |
| CC(1/2) | 0.998 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium chloride, 0.05M HEPES pH 7.6, 29% v/v pentaerythritol propxylate (5/4 PO/OH) |
