9LVE
Temperature induces a shift from the dihexamer to the hexamer form of insulin (300K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2024-09-17 |
| Detector | RIGAKU HyPix-Arc 150 |
| Wavelength(s) | 1.5 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.316, 78.316, 39.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.110 - 2.880 |
| R-factor | 0.3912 |
| Rwork | 0.319 |
| R-free | 0.38810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.380 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.110 | 2.983 |
| High resolution limit [Å] | 2.880 | 2.881 |
| Number of reflections | 1729 | 171 |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 83.8 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.947 | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium acetate trihydrate, 0.1M tris hydrochloride pH 9 |
