9LV3
Crystal structure of mutant H1 Haemagglutinin HN/18-HA FPP from Influenza A virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-05-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.782, 251.367, 113.103 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 43.610 - 2.700 |
| R-factor | 0.2349 |
| Rwork | 0.234 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.20.1-4487) |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 125.600 | 2.782 |
| High resolution limit [Å] | 2.500 | 2.503 |
| Rmerge | 0.240 | 0.640 |
| Number of reflections | 93542 | 35035 |
| <I/σ(I)> | 6.4 | 1.3 |
| Completeness [%] | 86.3 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Zinc acetate dihydrate, 20% w/v Polyethylene glycol 3,350 |
