9LV2

Crystal structure of H1 Haemagglutinin HN/18-HA2-L113S from Influenza A virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	65.091, 249.627, 65.509
Unit cell angles	90.00, 118.43, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.24376
Rwork	0.243
R-free	0.26651
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lxv
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (Phenix1.20.1-4487)
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.390	2.390
Rmerge	0.280	0.417
Number of reflections	42012	3655
<I/σ(I)>	5.2
Completeness [%]	80.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Potassium citrate tribasic monohydrate, 20% w/v Polyethylene glycol 3,350