9LUQ
Crystal structure of Pseudoalteromonas sp.L11 Tryptophan halogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 183.550, 183.550, 190.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.260 - 2.750 |
| R-factor | 0.1902 |
| Rwork | 0.190 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 7.723 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.260 | 2.750 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Number of reflections | 198825 | 9461 |
| <I/σ(I)> | 6.9 | 1 |
| Completeness [%] | 99.8 | |
| Redundancy | 21.1 | |
| CC(1/2) | 0.992 | 0.557 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.8M Sodium phosphate monobasic/1.2M Potassium phosphate dibasic, 100mM Sodium acetate/Acetic acid pH 4.5 |
