9LUA

Crystal structure of FIP200 Claw domain and SMCR8 FIR motif complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978510
Spacegroup name	H 3
Unit cell lengths	119.675, 119.675, 35.953
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.920 - 1.970
R-factor	0.2001
Rwork	0.198
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.699
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	2.010
High resolution limit [Å]	1.970	5.370	1.970
Rmerge	0.114	0.074	0.367
Rmeas	0.121	0.078	0.389
Rpim	0.039	0.025	0.128
Number of reflections	13359	663	685
<I/σ(I)>	12.5
Completeness [%]	99.6	99.7	100
Redundancy	10.1	10	8.8
CC(1/2)	0.990	0.996	0.935

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	Phosphate citrate, PEG 300,