9LS5

Entamoeba histolytica Cyclophilin 18 (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-08
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	37.401, 74.077, 62.813
Unit cell angles	90.00, 104.64, 90.00

Refinement procedure

Resolution	35.260 - 1.400
R-factor	0.1604
Rwork	0.159
R-free	0.18950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.894
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.260	1.420
High resolution limit [Å]	1.400	1.400
Number of reflections	62683	3084
<I/σ(I)>	9.6	2
Completeness [%]	96.2	96.2
Redundancy	2.2	2.2
CC(1/2)	0.996	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.1 M Sodium citrate tribasic dihydrate pH 5.6, 20% v/v 2-Propanol, 20% w/v Polyethylene glycol 4,000