9LRI

Crystal Structure of Thermus thermophilus KhpB/EloR complexed with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-16
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	35.261, 46.058, 56.885
Unit cell angles	97.34, 92.53, 112.69

Refinement procedure

Resolution	32.382 - 2.650
Rwork	0.204
R-free	0.26530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.172
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.990	2.750
High resolution limit [Å]	2.650	2.650
Rmerge	0.090	0.200
Rmeas	0.103	0.231
Rpim	0.031	0.088
Number of reflections	10994	599
<I/σ(I)>	30.1
Completeness [%]	97.7	97.3
Redundancy	8.3	7.5
CC(1/2)	0.999	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES (pH 7.5), 0.2M ammonium sulfate, 25% (w/v) polyethylene glycol 3350