9LRH

Killer immunoglobulin receptor KIR2DL2 in complex with KIR2DL2_KIR2DL2/3 agonist 61-scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	I 41 2 2
Unit cell lengths	98.855, 98.855, 403.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.380 - 3.400
R-factor	0.228
Rwork	0.227
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dl2
RMSD bond length	0.003
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.430	3.670
High resolution limit [Å]	3.400	3.400
Rmerge	0.209	3.364
Rmeas	0.217	3.489
Rpim	0.058	0.920
Total number of observations	193259	40580
Number of reflections	14337	2882
<I/σ(I)>	9.8	0.8
Completeness [%]	99.9
Redundancy	13.5	14.1
CC(1/2)	0.999	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	100mM BIS-TRIS propane pH7.0, 29% Tacsimate pH7.0