9LRG

Crystal Structure of Thermus thermophilus KhpB/EloR

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-19
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	47.011, 47.011, 265.782
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.990 - 2.990
R-factor	0.2515
Rwork	0.251
R-free	0.27020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.990	3.100
High resolution limit [Å]	2.990	2.990
Rmerge	0.142	0.275
Rmeas	0.145	0.145
Rpim	0.029	0.052
Number of reflections	3900	349
<I/σ(I)>	41.3
Completeness [%]	95.9	96.9
Redundancy	20	15
CC(1/2)	0.998	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2.0M sodium formate