9LRF

Killer immunoglobulin receptor KIR2DL2 in complex with KIR2DL2_KIR2DL2/3 agonist 61-Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9800
Spacegroup name	C 1 2 1
Unit cell lengths	170.109, 79.244, 64.364
Unit cell angles	90.00, 92.39, 90.00

Refinement procedure

Resolution	38.080 - 2.500
R-factor	0.2358
Rwork	0.234
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dl2
RMSD bond length	0.002
RMSD bond angle	0.524
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.330	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.069	1.085
Rmeas	0.082	1.273
Rpim	0.044	0.662
Total number of observations	99820	11977
Number of reflections	29367	3339
<I/σ(I)>	8.9	0.9
Completeness [%]	98.9
Redundancy	3.4	3.6
CC(1/2)	0.982	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	100mM imidazole-HCl pH7.5, 200mM Calcium acetate, 10% PEG3350