9LRA

Killer immunoglobulin receptor KIR2DL2 in complex with Pan2D Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.045
Spacegroup name	P 21 21 21
Unit cell lengths	79.253, 81.515, 123.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.200 - 2.600
R-factor	0.2215
Rwork	0.220
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dl2
RMSD bond length	0.002
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	25315	2496
<I/σ(I)>	10.7
Completeness [%]	100.0
Redundancy	13.7
CC(1/2)	0.996	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	293	200 mM Sodium Malonate pH5.0, 19% PEG3350