9LR7
Crystal Structure of the Basal pilin EbpB from Enterococcus faecalis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.307, 158.415, 88.221 |
| Unit cell angles | 90.00, 92.09, 90.00 |
Refinement procedure
| Resolution | 77.150 - 2.430 |
| R-factor | 0.22042 |
| Rwork | 0.219 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.383 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.150 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.100 | 0.635 |
| Rpim | 0.067 | 0.410 |
| Number of reflections | 74323 | 3716 |
| <I/σ(I)> | 7.4 | 2.1 |
| Completeness [%] | 93.5 | 75.6 |
| Redundancy | 3 | |
| CC(1/2) | 0.991 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 200mM Ammonium sulfate, 100mM Sodium acetate HCl, pH 4.6, 25% (w/v) PEG 4000 |
