9LPT

Structure of the P113 protein from Plasmodium falciparum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	291.15
Detector technology	PIXEL
Collection date	2017-03-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97914
Spacegroup name	P 31 2 1
Unit cell lengths	72.920, 72.920, 143.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.340 - 1.700
R-factor	0.1903
Rwork	0.189
R-free	0.22120
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.350
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.620	5.000
High resolution limit [Å]	1.700	1.700
Rmerge	0.129
Number of reflections	49135	49135
<I/σ(I)>	15.6
Completeness [%]	100.0
Redundancy	3.7
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.5 mM MES, 22% PEG3350