9LPG

Crystal structure of maize CRY-GL2 photosignaling complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	108.347, 217.056, 228.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.166 - 2.800
R-factor	0.2329
Rwork	0.232
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lz3
RMSD bond length	0.007
RMSD bond angle	1.168
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: 000))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.308	0.222	1.429
Rmeas	0.321	0.231	1.500
Rpim	0.088	0.064	0.447
Number of reflections	99342	3527	3192
<I/σ(I)>	3.8
Completeness [%]	99.7	99.9	96.4
Redundancy	13	12.8	10.7
CC(1/2)	0.975	0.970	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	300	20% (w/v) Polyethylene glycol 3,350, 100 mM Bis-tris Propane/Hydrochloric acid pH 6.5, 200 mM Sodium fluoride