9LPE
ATPDF2 HDZIP domain complexed with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.278, 73.278, 170.788 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.820 - 3.110 |
| R-factor | 0.276 |
| Rwork | 0.274 |
| R-free | 0.28240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.115 | 0.225 |
| Rmeas | 0.270 | |
| Rpim | 0.145 | |
| Number of reflections | 9792 | 935 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.973 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES monohydrate PH 6.0, 14% PEG 4000 |
