9LOM
Study on the structure and function of tumor suppressor ZMYND11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 198 |
| Detector technology | PIXEL |
| Collection date | 2022-11-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 55.022, 55.022, 74.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.650 - 1.630 |
| R-factor | 0.17358 |
| Rwork | 0.173 |
| R-free | 0.17784 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.760 | 1.660 |
| High resolution limit [Å] | 1.628 | 1.630 |
| Number of reflections | 8809 | 2301 |
| <I/σ(I)> | 73.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 19 | |
| CC(1/2) | 0.997 | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 40 % v/v 2-Methyl-2,4-pentanediol,100 mM MES pH 6.5 |
