9LOH

Crystal Structure of the Heterodimeric HIF-2 Complex with Agonist SD-10

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97907
Spacegroup name	P 1 21 1
Unit cell lengths	49.137, 98.469, 76.791
Unit cell angles	90.00, 107.10, 90.00

Refinement procedure

Resolution	73.400 - 3.020
R-factor	0.2331
Rwork	0.232
R-free	0.26220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.557
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.470	8.200	3.070
High resolution limit [Å]	3.020	6.510	3.020
Number of reflections	13668	696	708
<I/σ(I)>	11.5
Completeness [%]	99.0
Redundancy	6.8
CC(1/2)	0.996	0.996	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 3350, Succinic Acid pH 7.0