9LO2

Crystal Structure of Anti-CRISPR protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 63 2 2
Unit cell lengths	109.203, 109.203, 187.956
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.286 - 2.287
R-factor	0.2106
Rwork	0.208
R-free	0.24080
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.008
Data scaling software	HKL-2000 (v722)
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.287	4.950	2.300
Rmerge	0.299	0.160	3.055
Rmeas	0.304	0.163	3.129
Rpim	0.052	0.028	0.659
Number of reflections	30688	3355	2988
<I/σ(I)>	2.6
Completeness [%]	100.0	99.9	100
Redundancy	32.5	34.2	21.4
CC(1/2)	1.000	0.996	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Imidazole-HCl, pH 6.5, 0.9M Sodium citrate