9LNQ

The hUNG bound to DNA product embedding 4primer-OCH3-dU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-01-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	48.860, 65.339, 98.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.740 - 1.740
R-factor	0.1938
Rwork	0.192
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.740	1.802
High resolution limit [Å]	1.740	1.740
Rmerge		1.236
Number of reflections	402722	44792
<I/σ(I)>	25.69
Completeness [%]	92.4
Redundancy	13.1
CC(1/2)	1.000	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	20% polyethylene glycol 4000 (Sigma), 100 mM Hepes buffer (pH 6.5), 10% dioxane, 1 mM DTT