9LNK
Crystal structure of VANC21 in complex with its target DNA (native).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.640, 96.850, 167.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.350 - 2.550 |
| R-factor | 0.2228 |
| Rwork | 0.221 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 2.700 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmeas | 0.870 | 1.642 |
| Number of reflections | 28722 | 4424 |
| <I/σ(I)> | 19.64 | 1.51 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 9.26 | 8.82 |
| CC(1/2) | 0.999 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 9% PEG 3350, 75mM Hepes-KOH, pH 8.0, 50mM succinic acid |
