9LN8

Structure of NAP1 in complex with H2A.Z-H2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	66.852, 79.257, 175.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.060 - 2.800
R-factor	0.213
Rwork	0.211
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k0c
RMSD bond length	0.006
RMSD bond angle	0.979
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.107	0.045	0.642
Rmeas	0.116	0.049	0.703
Rpim	0.043	0.018	0.279
Total number of observations	154407
Number of reflections	23307	1244	1081
<I/σ(I)>	5.9
Completeness [%]	98.1	93.9	95.1
Redundancy	6.6	6.8	5.8
CC(1/2)	0.992	0.998	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG 3350