9LMM

An antibiotic biosynthesis monooxygenase family protein from Streptomyces sp. MA37

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	49.450, 59.840, 78.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.790 - 1.650
R-factor	0.2106
Rwork	0.210
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.307	1.700
High resolution limit [Å]	1.650	1.650
Number of reflections	28548	1890
<I/σ(I)>	21.9
Completeness [%]	99.2
Redundancy	10.7
CC(1/2)	0.999	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291.15	30% v/v PEG 300, 15% w/v PEG 1000, 0.1 M Bis-Tris propane at pH 7.0