9LMK

Room temperature structure of lysozyme by serial synchrotron crystallography (XDS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-02-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	79.180, 79.180, 38.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.990 - 1.500
R-factor	0.1848
Rwork	0.183
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wuc
RMSD bond length	0.007
RMSD bond angle	1.030
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	20136	1980
<I/σ(I)>	9.45
Completeness [%]	100.0
Redundancy	1124.7
CC(1/2)	0.994	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		295.5	Na-acetate, PEG 8000, NaCl