9LMG
Crystal structure of LooH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-03 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.294, 130.493, 168.474 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.200 - 1.630 |
| R-factor | 0.19398 |
| Rwork | 0.192 |
| R-free | 0.22285 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.120 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.700 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.052 | 0.517 |
| Rpim | 0.023 | 0.241 |
| Number of reflections | 143017 | 14132 |
| <I/σ(I)> | 30.33 | 2.88 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6 | 5.6 |
| CC(1/2) | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES, 25% PEG3350 |
