9LMA

Crystal structure of Ornithine decarboxylase H346A mutant without PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-26
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9734
Spacegroup name	P 1
Unit cell lengths	72.004, 72.307, 78.006
Unit cell angles	107.60, 114.56, 100.37

Refinement procedure

Resolution	29.290 - 1.740
R-factor	0.20392
Rwork	0.202
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.456
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.290	1.840
High resolution limit [Å]	1.740	1.740
Rmerge	0.238	1.130
Number of reflections	126051	17591
<I/σ(I)>	4.5
Completeness [%]	96.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium tartrate 10% v/v PEG3350, pH 6.6