9LLW
Crystal Structure of N-terminal flexible domain of the Shaft pilin EbpC from Enterococcus faecalis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 67.296, 67.296, 108.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.370 - 1.990 |
| R-factor | 0.18636 |
| Rwork | 0.183 |
| R-free | 0.24192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.518 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.370 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.072 | 0.600 |
| Rmeas | 0.073 | 0.610 |
| Rpim | 0.012 | 0.100 |
| Number of reflections | 9867 | 493 |
| <I/σ(I)> | 30.3 | 5.3 |
| Completeness [%] | 94.4 | 99.2 |
| Redundancy | 34.6 | 32.5 |
| CC(1/2) | 0.990 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 100mM CHES-NaOH, pH 9.5 30% (w/v) PEG 3000 |
