9LLS

Crystal structure of Ornithine decarboxylase H346A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-26
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97934
Spacegroup name	P 1
Unit cell lengths	72.364, 72.380, 78.050
Unit cell angles	114.65, 107.28, 100.49

Refinement procedure

Resolution	29.340 - 2.690
R-factor	0.17375
Rwork	0.171
R-free	0.23136
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.366
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.340	2.840
High resolution limit [Å]	2.690	2.690
Rmerge	0.127	0.268
Number of reflections	34924	4892
<I/σ(I)>	8.4
Completeness [%]	97.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium tartrate, 10% PEG3350 v/v pH 6.6