9LL6

Crystal structure of Ornithine decarboxylase H216F mutant without PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97933
Spacegroup name	P 1
Unit cell lengths	73.012, 73.120, 78.782
Unit cell angles	107.19, 114.42, 100.24

Refinement procedure

Resolution	44.540 - 2.640
R-factor	0.17201
Rwork	0.168
R-free	0.24186
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.417
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.790	2.790
High resolution limit [Å]	2.640	2.640
Rmerge	0.111	0.203
Number of reflections	36106	5256
<I/σ(I)>	8.1
Completeness [%]	97.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium tartrate dibasic, pH 6.6, 10% v/v PEG3350