9LL4
Crystal structure of the Helicobacter pylori copper resistance determinant CrdA in complex with silver ions in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-06-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.229, 39.026, 65.099 |
| Unit cell angles | 90.00, 102.11, 90.00 |
Refinement procedure
| Resolution | 29.840 - 1.970 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.735 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 17430 | 794 |
| <I/σ(I)> | 29.8 | 5.9 |
| Completeness [%] | 96.6 | 89.1 |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.990 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 291 | ammonium sulfate, citric acid |
