9LKG
Ornithine decarboxylase from Lacticaseibacillus rhamnosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.782, 80.607, 121.822 |
| Unit cell angles | 86.75, 80.61, 70.83 |
Refinement procedure
| Resolution | 47.250 - 2.090 |
| R-factor | 0.27891 |
| Rwork | 0.278 |
| R-free | 0.30103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.866 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.250 | 2.210 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.067 | 0.300 |
| Number of reflections | 137249 | 20787 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 96.6 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Ammonium tartrate dibasic,10% PEG3350, pH 6.6 |
