9LJX

Crystal Structure of the Heterodimeric HIF-2 Complex with Agonist SD-26

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	49.050, 97.634, 76.994
Unit cell angles	90.00, 106.98, 90.00

Refinement procedure

Resolution	28.540 - 2.800
R-factor	0.2259
Rwork	0.224
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	7.590	2.850
High resolution limit [Å]	2.790	6.030	2.800
Rmerge	0.078	0.052	0.750
Rmeas	0.084	0.056	0.816
Rpim	0.032	0.022	0.317
Number of reflections	17442	881	869
<I/σ(I)>	8.1
Completeness [%]	100.0	100	99.5
Redundancy	6.8	6.8	6.5
CC(1/2)	0.990	0.997	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 3350, Succinic Acid pH 7.0