9LJS

Structural insights into the polymerase catalyzed FAD-capping of hepatitis C viral RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2023-12-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	139.003, 139.003, 92.986
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.100 - 2.960
Rwork	0.177
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.350	2.910
High resolution limit [Å]	2.760	2.760
Number of reflections	26312	3749
<I/σ(I)>	8.1
Completeness [%]	99.7
Redundancy	12.5
CC(1/2)	0.984	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	PEG 3350, 100 mM Bis-Tris propane/hydrochloric acid, pH 8.5, 200 mM Sodium malonate dibasic precipitant