9LJK
Structure of the periplasmic domain of MotS from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.990, 80.730, 55.240 |
| Unit cell angles | 90.00, 100.44, 90.00 |
Refinement procedure
| Resolution | 54.330 - 1.890 |
| R-factor | 0.1765 |
| Rwork | 0.174 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.865 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.330 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.090 | |
| Number of reflections | 28678 | 1861 |
| <I/σ(I)> | 8.7 | 2.3 |
| Completeness [%] | 99.3 | 99.8 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.993 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-NaOH pH7.5, 1.26 M ammonium sulfate, protein conc 6 mg/mL |
