9LJ6
Crystal Structure of C-terminal rigid fragment containing middle and C-terminal domains of the Basal pilin EbpB from Enterococcus faecalis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-08 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 118.538, 118.538, 69.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 102.870 - 2.160 |
| R-factor | 0.1619 |
| Rwork | 0.161 |
| R-free | 0.19015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.701 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.870 | 2.410 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rpim | 0.036 | 0.330 |
| Number of reflections | 17032 | 851 |
| <I/σ(I)> | 12.4 | 1.9 |
| Completeness [%] | 94.4 | 75.4 |
| Redundancy | 9.4 | |
| CC(1/2) | 0.990 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 200mM Na/K Phosphate, 100mM Bis-tris pH 7.5, 20% (w/v) PEG 3350 |
