Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2024-06-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.422, 77.159, 78.099 |
| Unit cell angles | 90.00, 97.98, 90.00 |
Refinement procedure
| Resolution | 34.870 - 3.150 |
| R-factor | 0.2478 |
| Rwork | 0.243 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c3t |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.646 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.870 | 3.263 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 27821 | 1454 |
| <I/σ(I)> | 8.37 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.993 | 0.859 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M Sodium phosphate dibasic dihydrate,pH 9.1 20% w/v Polyethylene glycol 3,350 |
