9LIN

Crystal structure of apo dGCA1_L83A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 63
Unit cell lengths	84.488, 84.488, 67.615
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.240 - 1.850
R-factor	0.16
Rwork	0.158
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.240	1.916
High resolution limit [Å]	1.850	1.850
Rmerge	0.206	1.525
Number of reflections	23455	9493
<I/σ(I)>	12.77
Completeness [%]	99.3	95.94
Redundancy	10.9	1.79
CC(1/2)	0.993	0.270

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.09 M NPS, 0.1 M Buffer System 3 8.5, 30 % v/v Precipitant Mix 2