9LIE

Repetitive domain (RP) 2 structure of aciniform spidroin from Latrodectus hesperus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	33.875, 57.343, 66.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.200 - 1.060
R-factor	0.2053
Rwork	0.205
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.467
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.460	1.110
High resolution limit [Å]	1.060	1.060
Rmerge	0.057
Rmeas	0.059
Rpim	0.017
Number of reflections	57444	7165
<I/σ(I)>	18.4
Completeness [%]	95.9	84.2
Redundancy	11.6
CC(1/2)	0.997	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M Tris8.0 30% w/v Polyethylene glycol monomethyl ether 2000