9LIC
Crystal structure of apo form of protein tyrosine phosphatase 1B (PTP1B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2014-12-06 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.2833 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.510, 61.100, 88.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.790 - 1.900 |
| R-factor | 0.1873 |
| Rwork | 0.186 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.793 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.510 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.330 |
| Number of reflections | 24952 | 1444 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.01M HEPES pH 8.5, 30% (w/v) PEG 3350, 0.2M MgCl2 |
