9LHR
Crystal structure of Ice binding protein from Candidatus Cryosericum odellii SMC5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.750, 47.560, 66.770 |
| Unit cell angles | 90.00, 92.22, 90.00 |
Refinement procedure
| Resolution | 29.394 - 1.800 |
| Rwork | 0.163 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.674 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.394 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.180 |
| Number of reflections | 63299 | 3705 |
| <I/σ(I)> | 25.6 | |
| Completeness [%] | 98.6 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium cacodylate pH 5.0, 0.2 M Sodium acetate trihydrate |
