9LHG

Crystal structure of BglB with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 21 2 21
Unit cell lengths	90.240, 99.129, 116.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.123 - 1.797
R-factor	0.1646
Rwork	0.163
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.460	1.890
High resolution limit [Å]	1.797	1.800
Number of reflections	93489	14092
<I/σ(I)>	15.4
Completeness [%]	95.6
Redundancy	8.3
CC(1/2)	0.999	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1M Sodium acetate trihydrate pH 4.77 10% (w/v) PEG8000