9LGS

R-degron fused ZZ-domain of the Arabidopsis thaliana E3 ubiquitin-protein ligase BIG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00
Spacegroup name	C 2 2 2
Unit cell lengths	79.747, 146.855, 48.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.710 - 1.500
R-factor	0.1757
Rwork	0.175
R-free	0.18460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.209
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.099	0.728
Number of reflections	46158	46148
<I/σ(I)>	25.6	2
Completeness [%]	99.2
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% w/v Polyethylene glycol 3,350 0.1 M HEPES sodium pH 7.0 0.01 M Magnesium chloride hexahydrate 0.005 M Nickel(II) chloride hexahydrate