9LGQ

The crystal structure of SARS-CoV-2 NSP5 in complex with PTBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-21
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	96.154, 81.651, 54.334
Unit cell angles	90.00, 116.99, 90.00

Refinement procedure

Resolution	32.760 - 1.820
R-factor	0.1954
Rwork	0.194
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	6.452
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.760	1.885
High resolution limit [Å]	1.820	1.820
Number of reflections	64657	32842
<I/σ(I)>	24.55
Completeness [%]	97.8
Redundancy	6.9
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M MES pH 5.6, 7% PEG 6000, and 6% dimethyl sulfoxide (DMSO)