9LGG

Crystal structure of the human MuSK(Ig3_Fz)/L1 peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999
Spacegroup name	P 64
Unit cell lengths	103.644, 103.644, 305.216
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.680 - 2.690
R-factor	0.2059
Rwork	0.204
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.636
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.070	2.860
High resolution limit [Å]	2.690	2.690
Number of reflections	50770	8124
<I/σ(I)>	10.38	1.38
Completeness [%]	99.4	98.5
Redundancy	4.51
CC(1/2)	0.997	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Na-Acetate(pH 6.0), 0.2M NaCl, 7% PEG 8000, 10% Glycerol