9LFS

Crystal of Glucosidase form Ophiorrhiza pumila

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	277.15
Detector technology	PIXEL
Collection date	2024-08-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97923
Spacegroup name	I 4 2 2
Unit cell lengths	137.755, 137.755, 164.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.930 - 1.950
R-factor	0.1799
Rwork	0.179
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.941
Data reduction software	autoPX
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.930	2.019
High resolution limit [Å]	1.949	1.949
Number of reflections	57388	5694
<I/σ(I)>	14.36
Completeness [%]	99.2
Redundancy	1.993
CC(1/2)	0.999	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	Consistent with the conditions under which loganin was added, the reservoir solution consisted of 0.1 M sodium acetate trihydrate at pH 4.6 and 2.0 M ammonium sulfate. The sessile drop plate was covered with an adhesive tape and incubated in a cabinet maintained at a temperature of 291.15 K. Diffraction-quality crystals were produced within a time-frame of 10 to 15 days.