9LF8

Structure of MPXV M1R and mMM1-16 Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 43
Unit cell lengths	177.378, 177.378, 90.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.340 - 2.700
R-factor	0.2051
Rwork	0.202
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.700
High resolution limit [Å]	2.700	2.700
Number of reflections	150678	24333
<I/σ(I)>	11.23
Completeness [%]	99.9
Redundancy	5.3
CC(1/2)	0.996	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M ammonium fluoride, 20% w/v polyethylene glycol 3,350